Fusarium wilt of banana, also known as "banana cancer", currently threatens banana production areas worldwide. Timely and accurate identification of Fusarium wilt disease is crucial for effective disease control and optimizing agricultural planting structure. To explore the use of unmanned aerial vehicle (UAV) remote sensing for identifying banana wilt disease, this study obtained comprehensive experimental data on wilted banana plants in a banana plantation in Long'an County, Guangxi. The data set includes UAV multispectral reflectance data and ground survey data on the incidence of banana wilt disease. The UAV multispectral imaging data have high spatial resolution, while the ground survey data is accurate, making them suitable for research on UAV remote sensing identification and monitoring of banana wilt disease. This data set is of great significance for promoting research and application of remote sensing monitoring of crop diseases. Fusarium wilt of banana, also known as "banana cancer", currently threatens banana production areas worldwide. Timely and accurate identification of Fusarium wilt disease is crucial for effective disease control and optimizing agricultural planting structure. To explore the use of unmanned aerial vehicle (UAV) remote sensing for identifying banana wilt disease, this study obtained comprehensive experimental data on wilted banana plants in a banana plantation in Long'an County, Guangxi. The data set includes UAV multispectral reflectance data and ground survey data on the incidence of banana wilt disease. The UAV multispectral imaging data have high spatial resolution, while the ground survey data is accurate, making them suitable for research on UAV remote sensing identification and monitoring of banana wilt disease. This data set is of great significance for promoting research and application of remote sensing monitoring of crop diseases.