The management of multi-use landscapes is challenging, but essential when aiming at preserving the potential for ecosystem service provision. Land-use decisions lay at the center of this challenge. While land-use decision models may help to transparently grasp land-use decisions, the parameterization of such models is difficult as human decision-making is often not rational. We show here how we used a serious game to parameterize a Bayesian network-based land-use decision model. To elicit validation, game outputs are transformed to conditional probabilities and compared to conditional probabilities parameterized via a questionnaire and workshop exercises. The analysis of four types of validity shows encouraging results for criterion, respondent-related and practice-related validation. However, content validation (sensitivity analysis) was disappointing initially. We discuss how the success in validation quality may be related to the design of the game and conclude that the transfer from a game to Bayesian networks could improve the parameterization quality. Environmental Modelling & Software, 122 ISSN:1364-8152 ISSN:1873-6726