Ninety-nine dwarf and photoperiod-insensitive breeding lines developed from Ethiopian sorghum germplasm were inoculated with Colletotrichum sublineolum and evaluated for anthracnose resistance at the Tropical Agriculture Research Station in Isabela, Puerto Rico, during the 2008 and 2009 growing seasons. A resistant response was observed for 57 lines in 2008 and for 47 lines in 2009 with 44 lines showing a resistant response across growing seasons. These lines showed reddening of inoculated leaves and no acervuli. Variation in resistance across growing seasons and replications within a growing season was observed for 19 lines rated as susceptible and these lines also showed low disease severity. Mean disease severity for the susceptible accessions was similar across growing seasons with a mean of 26% for 42 lines in 2008 and 23% for 52 lines in 2009. The resistant lines showed phenotypic diversity in response to anthracnose, suggesting potential genetic variation for resistance. Accepted for publication 31 October 2010. Published 23 November 2010.