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Comparison of Two Bacterial Characterization Techniques for the Genomic Analysis of River Microbiome

Domain:

environment and energy
Creator:
RobVicIreArg
Publisher:
MDP
Host:
We compared the performance of matrix-assisted laser desorption/ionization followed by a time of flight (MALDI-TOF) mass spectrometry and genomic DNA extraction followed by sequencing, assembly, and alignment for phylogenetic assessment (Genomics method). We collected the samples from four contaminated rivers in the Dominican Republic and analyzed MALDI-TOF efficacy and accuracy for identifying bacteria in the samples. We evaluated the results for both methods (MALDI-TOF and Genomics) and reported a similarity percentage between each method's results. The MALDI-TOF method had a 72.41\% of coincidence with the Genomics method. This could have been sequence contamination found in the Genomics method. When it was later filtered, the result's coincidence rate went up to 90\%.

Visit

doi.org

Licenses

http://creativecommons.org/licenses/by/4.0

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