Data from: Reproductive isolation and local adaptation quantified for a chromosome inversion in a malaria mosquito

Chromosome inversions have long been thought to be involved in speciation and local adaptation. We have little quantitative information, however, about the effects that inversion polymorphisms have on reproductive isolation and viability. Here we provide the first estimates from any organism for the total amount of reproductive isolation associated with an inversion segregating in natural populations. We sampled chromosomes from 751 mosquitoes of the malaria vector Anopheles funestus along a 1421 km transect in Cameroon that traverses savannah, highland, and rainforest ecological zones. We then developed a series of population genetic models that account for selection, migration, and assortative mating, and fit the models to the data using likelihood. Results from the best-fit models suggest there is strong local adaptation, with relative viabilities of homozygotes ranging from 25% to 130% compared to heterozygotes. Viabilities vary qualitatively between regions: the inversion is underdominant in the savannah, while in the highlands it is overdominant. The inversion is also implicated in strong assortative mating. In the savannah, the two homozygote forms show 92% reproductive isolation, suggesting that this one inversion can generate most of the genetic barriers needed for speciation. KaryotypeCountsField data. Number of Anopheles funestus collected in populations of Cameroon, by the type of arrangement in3Ra. Columns are: The name of the village, Longitude and Latitude (in WGS84 UTM Zone 33 North), the distance in km from the start of the transect, the counts of Standard homozygotes (SS), Heterozygotes (SI), and Inverted Homozygotes (II), and the total of individuals collectedSource CodeCode.zip