This study investigated the effectiveness of demonstration and instructional analogy teaching
methods in enhancing secondary school students' interest in Biology in Delta State, Nigeria. The
study adopted a quasi-experimental pretest–posttest non-equivalent control group design
involving two experimental groups (demonstration and instructional analogy) and one control
group (lecture method). The population comprised 39,904 Senior Secondary School Two (SS II)
Biology students in 474 public mixed secondary schools in Delta State. A sample of 298 students
drawn from six public mixed secondary schools was selected using a stratified random sampling
technique. Data were collected using the Biology Interest Scale (BIS), which yielded a Cronbach
Alpha reliability coefficient of 0.78. Mean and standard deviation were used to answer the research
questions, while Analysis of Covariance (ANCOVA), Scheffé post hoc test, and independent
samples t-test were used to test the hypotheses at the 0.05 level of significance. The findings
revealed a significant difference in the interest scores of students taught Biology using
demonstration, instructional analogy, and lecture methods, with the demonstration method
producing the highest level of students' interest. The Scheffé post hoc analysis further showed that
the demonstration method was significantly more effective than both instructional analogy and
lecture methods, whereas no significant difference existed between instructional analogy and
lecture methods. The study also found no significant gender differences in the interest scores of
students taught using either the demonstration or instructional analogy method. The study
concluded that the demonstration method is the most effective instructional approach for
enhancing students' interest in Biology. It was recommended that Biology teachers should adopt
demonstration and instructional analogy teaching methods to promote students' interest in
Biology.