A three-pronged, locally rooted biomedical informatics (BMI) capacity strengthening approach contributes to the transformation of BMI education and research in Mozambique. This program integrates faculty fellowships, a new BMI track in a master’s program, and annual workshops and bootcamps to drive sustainable change, improve practitioners’ BMI skills, and strengthen institutional research capacity. Continuous evaluation is integral to program success. Multiple evaluation methods were used to assess implementation and outcomes. Pre- and post-workshop surveys measured changes in knowledge and skills. Independent samples t-tests assessed differences between pre- and post-training responses. Course evaluations and exit focus groups obtained feedback about the fellowship. Findings indicated training efficacy and growth in BMI knowledge and research skills. Integrated, continuously evaluated BMI training can contribute to sustainable workforce and research capacity development in sub-Saharan Africa.