Evidence of dengue virus transmission and a diverse Aedes mosquito virome on the Democratic Republic of Congo-Angola border_R script for phylogenetic_tree_visualization

The code in this project accompanies an effort to investigate the virome of wild-caught Aedes mosquitoes from Kimpese, the Democratic Republic of the Congo (DRC). The DRC is the second largest country in Africa and has an estimated population of over 100 million. Its tropical climate is conducive to mosquito survival. Considerable attention is devoted to the high burden of malaria in the DRC, but arboviruses remain neglected, with limited studies of humans and mosquitoes to-date. To improve our understanding of the role of Aedes mosquito vectors in arboviral transmission in the DRC, we collected and sequenced the virome of mosquito pools from three areas of Kimpese, a region near the Angola border which has experienced recent arboviral outbreaks. Full details of this project can be found within this pre-print: Evidence of dengue virus transmission and a diverse Aedes mosquito virome on the Democratic Republic of Congo-Angola border (DOI: Evidence of dengue virus tr…). Details of the code are available at: github.com (*In our GitHub folder, we used ggtree (version 3.16.0); the code is slightly different from this capsule but produces the same plots.) Overview of the scripts found here: Visualization of phylogenetic analysis.R: This script can be executed using the example data files. *# R script rely on functioning installations of R software (version 4.5.1) and RStudio (version 2025.05.1). Installing R and RStudio typically takes about 10–30 minutes, depending on your computer's speed, operating system, and familiarity with the process. Running of the "Phylogenetic tree visualization.R" on the example data files takes up to 30 minutes.