rhparry/BtTV4a

This repository contains supplementary data for the manuscript "Bemisia tabaci toursvirus (BtTV-4a), a ubiquitous dsDNA virus in African and North American whiteflies, expands the toursviruses to Hemipterans." # BtTV-4a: Bemisia tabaci toursvirus 4a This repository contains supplementary data for the manuscript "Bemisia tabaci toursvirus (BtTV-4a), a ubiquitous dsDNA virus in African and North American whiteflies, expands the toursviruses to Hemipterans." ## Overview BtTV-4a is the first toursvirus identified in a hemipteran insect. This discovery significantly expands our understanding of ascovirus host range and evolution. The genome represents a large circular viral DNA sequence of 186,646 bp containing 200 predicted open reading frames (ORFs). ## Genome Information - **Accession number**: OM899612 - **Genome size**: 186,646 bp - **GC content**: 32.3% - **Number of ORFs**: 200 - **Raw sequencing data**: Available at NCBI SRA under BioProject ID PRJNA418329 ## Supplementary Files ### Supplementary File 1: BtTV-4a protein annotation Complete annotation of the 200 predicted ORFs in the BtTV-4a genome, including protein domains and functional predictions based on Pfam and Conserved Domain Database analyses. ### Supplementary File 2: Protein sequences for phylogeny The sequences of ten conserved viral proteins from representatives of Ascoviridae and Iridoviridae families used in the phylogenetic analysis. ### Supplementary File 3: Unaligned protein sequences Raw protein sequences of conserved genes from representative viruses before multiple sequence alignment, used for phylogenetic analyses. ### Supplementary File 4: MAFFT aligned sequences Multiple sequence alignments of conserved viral proteins used for phylogenetic tree construction. These alignments were generated using MAFFT and used as input for IQ-TREE2. ### Supplementary File 5: Orthogroup annotation Orthologous cluster analysis results showing shared gene families between BtTV-4a and other Ascoviridae members, generated using OrthoFinder. ## Citation If you use these data in your research, please cite: Parry, R.H., Wosula, E.N., Wilson, J.R., Chanda, B., Hasegawa, D., Gilliard, A., Asgari, S., Fei, Z. …