HBV genotyping by multiplex nested PCR v1

Hepatitis B virus is hyperendemic in Nigeria with a national seroprevalence of 12.2%. Worldwide, 10 genotypes have been identified on the basis of ≤8% difference in genome sequences, each with a distinctive geographical distribution. Available literature reveal genotype E as being predominant in West Africa. This study aimed at identifying the current pattern and prevalent genotypes of HBV in Zaria, a prominent and ancient region of northern Nigeria. Four milliliter of blood was collected in EDTA-container from each of 165 HBsAg-positive participants recruited consecutively. Plasma was separated and frozen at -20oC till analysis. Multiplex- nested PCR using type-specific primers was used to identify the various HBV genotypes.