Immunofluorescent FITC labelling of the MSPDBL2 antigen.

(A) Immunofluorescent FITC (green) labelling of the MSPDBL2 antigen (product of PF10_0355) in a minority of schizonts with individual microscopic fields illustrated for three parasite isolates (HB3, K1 and D6), alongside staining of parasite DNA by DAPI (blue) for the same fields. Parasite immunofluorescence shows reactivity with 1/500 diluted mouse antiserum raised to a recombinant protein representing the N-terminal of MSPDBL2. (B) Proportions (with 95% CI) of schizonts positive for DBLMSP2 in 12 cultured isolates each with a different single parasite genotype. (C) Proportions of schizonts positive for DBLMSP2 in eight sub-clones of clone HB3. Exact numbers counted are shown in Table S3.