We plotted the first two dimensions of a multidimensional scaling analysis of pairwise identity by state across all study populations. (A) The HOA populations are broadly scattered between out-of-African populations and the bulk of sub-Saharan African populations along the first dimension. Some clusters of HOA individuals are much closer to the main sub-Saharan African cluster, while others are much closer to North African and Arabian clusters. (B) In this plot, we zoom in on the HOA samples and leave out all other populations. While the region as a whole covers a broad swath of the first MDS dimension, most individual populations are tightly clumped, with groups separated by language. The Nilo-Saharan speaking Gumuz are on the far left, the Omotic speaking Ari are in the center, and the Cushitic and Semitic speaking populations are on the right.