This database contains 100 (5 clones scanned 2 times) NIR spectra of cassava flour acquired in NRCRI (Nigeria), by using ASD QualitySpec NIR spectrometer. The spectra correspond to 50 genotypes (of WP4 clones) from Advanced Yield Trial (AYT) and Uniform Yield Trial (UYT) of NEXTGEN Cassava Breeding Project (NRCRI), Umudike NIGERIA in November 10, 2021.