Zoonotic infectious diseases have been an important concern to humankind for more than 10,000 years. Today, approximately
75% of newly emerging infectious diseases (EIDs) are zoonoses that result from various anthropogenic, genetic, ecologic,
socioeconomic, and climatic factors. Zoonotic EIDs remain a major global concern, and such threats are expanding, especially
in less developed regions. Current Ebola epidemic in West Africa is an extreme stark reminder of the role animal reservoirs
play in public health, which reinforces the urgent need to operationalize a One Health approach. Building opportunities to
overcome the challenges largely depends on four key capacity-building needs: (1) development of adequate science-based
risk management policies, (2) skilled-personnel capacity building, (3) accredited veterinary and public health diagnostic
laboratories with a shared database, and (4) improved use of existing natural resources and implementation. This paper
highlights the key issues as building block for synthesis of the One Health approach to address the challenges and opportunities
for tackling infectious diseases at the human, animal, and environment interface in low-resource settings. Health laboratory
services are essential for the efficient delivery of quality and cost-effective healthcare. Training in laboratory services has long
been a neglected part of the one health approach in countries like Sierra Leone. Njala University is actively working with other
partners of Royal Netherlands Embassy (RNE) Post-Ebola Resilience Project, further reinforcing the One Health Concept by
focusing on both human and animal health and its interface.