The use of geo-electrical resistivity survey to identify water bearing zones (aquifers) in porous rocks is trivial, but the delineation of groundwater quality using the same scheme still remains a predominantly subjective exercise with peculiarities that may vary from locality to locality. 2-D geoelectrical resistivity modelling with acquisition/interpretational parameter specification for localized groundwater-iron prediction was carried out in selected transition aquifers in Bayelsa State, Southern Nigeria. The aim of the study was to predict the concentration of iron in groundwater by specifying parameters for its direct interpretation from 2-D resistivity survey using integrated geoelectrical and physicochemical data in the study area. It was observed that an index resistivity value greater than 200 Ωm (≥ 200 Ωm) could be tied to iron (II) Fe2+ concentrations less than the WHO limit of 0.3 mg/lit indicative of best groundwater quality in the study area. Also, a 2-D resistivity survey dipole spacing of 2.5 m and depth of investigation of about 12.5 m using a 21 electrode system in a combined Wenner-Schlumberger Configuration was recommended for near surface groundwater aquifer exploration in the survey area.