Phylogenetic tree depicting HIV sequences from Maputo analyzed in 2009 TDR threshold survey.

Tree was constructed bases on complete sequences of protease and part of RT gene (1.317 bp), using Kimura 2-parameter (K2P) model of base substitution with bootstrap analysis (100 replications) in MEGA 5.05. Nomenclature of samples herein characterized is as follows: MZ (country) and year of sequencing-patient code-region of isolation [CID: Jose Macamo Health Center and 1° de Junho Health Center]. Tree includes sequences of the HIV-1 subtypes inferred through REGA (empty circles) along with previously described subtype C in the region, India and Brazil (full circles), other subtypes (other full shapes) and outlier group CPZ, obtained from Los Alamos HIV Sequence Database Subtype Reference Alignments (http: //www.hiv.lanl.gov/content/in…).