A Gram-negative rod with a single polar flagellum was isolated from a freshwater reservoir used for household purposes in Boane District, near Maputo, Mozambique, and designated as strain DB1 T . Growth was observed at 30–42 °C (optimum, 30–37 °C) and with 0.5–1.5 % NaCl. Whole-genome-, rpoD- and 16S rRNA-based phylogenies revealed this isolate to be distant from other
Pseudomonas
species with
Pseudomonas resinovorans
,
Pseudomonas furukawaii
and
Pseudomonas lalkuanensis
being the closest relatives. Phenotypic analyses of strain DB1 T showed marked differences with respect to type strains
P. resinovorans
CCUG 2473 T ,
P. lalkuanensis
CCUG 73691 T ,
P. furukawaii
CCUG 75672 T and Pseudomonas otiditis CCUG 55592 T . Taken together, our results indicate that strain DB1 T is a representative of a novel species within the genus
Pseudomonas
for which the name Pseudomonas boanensis is proposed. The type strain is DB1 T (=CCUG 62977 T =CECT 30359 T ).