A M. ulcerans isolates from the Densu river basin of Ghana were analyzed at 65 SNP loci (Primer IDs) by real-time PCRs. Base exchanges relative to the reference sequence of strain Agy99 were registered as 1 (grey). Allele matches with Agy99 were recorded as 0 (white). 5 haplotypes in addition to haplotype 1 (Agy99) could be distinguished on the basis of 14 SNP loci. B SNP typing results of strains from a second BU endemic area of Ghana as well as from additional African countries carried out with the set of SNP assays developed by whole genome sequencing of Ghanaian isolates. AW: Amansie West; Ga: strains from the Densu river basin; IC: Ivory Coast; T: Togo; B: Benin; C: Democratic Republic of Congo; A: Angola.