An interventional research was conducted in the kitchens of Khartoum state hospitals 2021 on the impact of microbial quality in (12) hospital. Samples taken from meals, hands, environment, surfaces, equipment, and utensils before and after interventions and measuring results. Questionnaires were filled out with interview with nutrition supervisor about kitchens. The data was statistically analyzed by using the Statistic Package for Social Sciences (SPSS) program version 25; the Paired Samples T Test and Chi-Square Test was used and there is an improvement at the level of indication 0.000 at the level (0.05). The results showed that the lack of quality of kitchens in buildings, equipment, utensils, hand facilities and no periodic inspection for raw materials. The study concluded that taken samples has an effective impact on microbial quality. The study recommended that the ministry of health and hospitals to commitment to take samples periodically.