Ultrafast Assessment of the Presence of a High-Risk Mycobacterium tuberculosis Strain in a Population
Domain:
healthcareCreator:
LauMarIñaMar
Publisher:
Ame
Host:
ABSTRACT
A persistent 8-year infection by a Beijing
Mycobacterium tuberculosis
strain from a previous outbreak after importation from West Africa obliged us to investigate secondary cases. We developed a multiplex PCR method based on whole-genome sequencing to target strain-specific single nucleotide polymorphisms (SNPs). In 1 week, we analyzed 868 isolates stored over 6 years. Only 2 cases (immigrants from Guinea Conakry) harbored the strain, which ruled out transmission—despite opportunities—and challenged some of the advantages associated with Beijing strains.
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