Pie charts represent disseminated infection (DI), midgut escape barrier (MEB), and midgut infection barrier (MIB). The DI rate is the number of mosquitoes with virus in the head/thorax or legs divided by the total number of mosquitoes that bloodfed. The MEB rate is the number of mosquitoes without virus in the head/thorax or legs divided by the total number of midgut infected mosquitoes, and MIB is the number of mosquitoes without virus in the midgut divided by the total number of bloodfed mosquitoes.