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A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes-1

Domaine:

healthcare

Type de record:

dataset
Créateur:
WouTerCorJea
Hôte:avatar

Copyright information:

Taken from "A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes"

BMC Biotechnology 2004;4():6-6.

Published online 1 Apr 2004

PMCID:PMC406508.

Copyright © 2004 van Wyngaardt et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

Phages were selected by panning on immobilised proteins, haptens and viruses. Pools released from the affinity matrix after each round of selection were amplified, purified by precipitation and tested for binding to the homologous target and several heterologous antigens: BSA = bovine serum albumin, MP = 2% milk powder in PBS, MBP = maltose binding protein, F-BSA = fluorescein-conjugated bovine serum albumin, N-BSA = 4-hydroxy-3-iodo-5-nitrophenylacetic acid conjugated to bovine serum albumin, AHSV = purified African horsesickness virus particles, BTV = purified bluetongue virus particles, CMV = purified cucumber mosaic virus particles, CYT = cytochrome C, THY = porcine thyroglobulin, CHY = chymotrypsinogen, KLH = keyhole limpet haemocyanin, TEL = turkey egg lysozyme. The homologous antigens in each case were: A) F-BSA, B) N-BSA, C) KLH, D) TEL, E) CYT, F) THY, G) CMV, H) AHSV and I) BTV. Figures on the X axis indicate the panning round of which the output was tested for polyclonal binding. The figure 0 refers to the unpanned library.

Visit

figshare.com

Tags

Genetics not elsewhere classifiedsemisyntheticsinglechainphagedisplaylibrarybasedchickenimmunoglobulinGenetics

Licenses

CC BY 4.0

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