Additional file 1: Fig. S1. Maximum likelihood tree of Anopheles ITS2 sequences. Seaview v4 software, Clustal W and phyML tools. Specimens identified in reference centres are indicated by taxonomic identification along with GenBank accession number, namely, An. arabiensis (Pretoria University, South Africa), An. gambiae (IRD Montpellier, France) and An. funestus (MRTC Bamako, Mali).