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Additional file 4: Figure S1. of A single-nucleotide-polymorphism real-time PCR assay for genotyping of Mycobacterium tuberculosis complex in peri-urban Kampala

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LRPS is more sensitive than LSP-PCR typing: H37Rv genomic DNA was serially diluted and analyzed with RD 724 (N = 10) or RD 750 (N = 10) deletion primers: lane M = molecular weight markers, lane 1 positive control (MTB Uganda family or lineage 3 strain), lane 2 negative control, lane 3- 12 H37Rv DNA diluted from 100-10 ng; the minimum dilution of genomic DNA that can be amplified was in lane 10 (30ng) (Plate a & c). Plate b & d are the corresponding LRPS using Rv004c/probe or Rv0129c/probe set with H37Rv genomic DNA diluted from (10-1) ng per assay, the minimum dilution of DNA that can be amplified was 3ng (See arrow on the amplification curves plate b & d). (DOC 179 kb)

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BiophysicsBiochemistryMicrobiologyFOS: Biological sciencesFOS: Biological sciencesGeneticsMolecular Biology39999 Chemical Sciences not elsewhere classifiedFOS: Chemical sciencesFOS: Chemical sciences+7

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