Trichomoniasis is one of the most common non-viral sexually transmitted infections worldwide; however, awareness and knowledge about this disease remains limited in many countries, particularly in developing regions. This study aimed to assess the level of knowledge and awareness of trichomoniasis among women in Western Libya. A descriptive-analytical study was conducted among 200 women recruited from Surman, Sabratha, Abu Issa, and Zawia. Data were collected using a structured questionnaire covering demographic characteristics, awareness, and knowledge of transmission, symptoms, complications, and prevention practices. Statistical analysis was performed using SPSS, with chi-square tests applied to assess associations. A p-value < 0.05 was considered statistically significant. The results revealed a low level of awareness, with only 28% of participants having heard of trichomoniasis. Knowledge of the causative agent was limited, as only 19% correctly identified it as a parasitic infection. Misconceptions were common regarding transmission, with many participants incorrectly attributing it to poor hygiene or sharing personal items. Awareness of symptoms and complications was also inadequate, and nearly half of the participants were unaware that the infection can be asymptomatic. Preventive knowledge was poor, with 44.5% unable to identify any preventive measures. Education level was significantly associated with both awareness (P = 0.015) and health-seeking behavior (P = 0.001), while age and residence showed no significant association. Despite low awareness, 54.5% of participants expressed willingness to seek medical care, and 65% showed interest in receiving more information. The study demonstrates a significant gap in awareness and knowledge regarding trichomoniasis among women in Western Libya. Targeted health education and awareness programs are urgently needed to enhance early diagnosis, effective treatment, and reduction of infection.