Background: Pesticides application for controlling pests, has been a matter of debate for long time. In order to guarantee sustainable human health, this application should be monitored continuously. Materials and Methods: 65 samples of cattle milk were collected from different Khartoum state’s municipals; to assess the OCP residue, samples were extracted with petroleum ether and cleaned up using silica gel, then read out by GC equipped with FID. Result: Two pesticides belong to OCP were detected, Endosulfan (69.8%) and Heptachlor (92%); the detected concentration of the second one was outstanding, as all positive samples were above the MRL.