Bat IRF7 interacts with bat MyD88. (A) Binding of bat IRF7 to bat MyD88.
Domaine:
healthcare
HEK293T cells were transfected with GFP-tagged IRF7 and FLAG-tagged MyD88 (human or bat, as indicated). At 24 h post-transfection, whole cell lysate was prepared and immunoprecipitated with anti-FLAG antibody M2. Immunoprecipitated complexes (IP) were analysed by immunoblot for IRF7 and MyD88 expression using an anti-human IRF7 antibody (top panel), and an anti-FLAG antibody M2 for detection of FLAG tagged MyD88 (middle panel). Whole cell lysate (10 ul) was also run on an SDS-PAGE gel and subsequently analysed for IRF7 expression using anti-human IRF7 antibody (bottom panel). (B) Bat IRF7 co-localizes with bat MyD88. PaKiT03 cells were transfected with bat GFP-IRF7/IRF3 and bat FLAG-MyD88 and HEK293T cells were transfected with human GFP-IRF7 and human or bat FLAG-MyD88. 16 hours post-transfection (or 24 h), cells were fixed for indirect immunofluorescence assay using an anti-human MyD88 antibody and red fluorescence-conjugated secondary antibody. Pictures show co-localisation of bat IRF7 or bat IRF3 with bat MyD88 in PaKiT03 cells and human IRF7 or human IRF3 with human MyD88 in HEK293T cells.