The persistent decline in junior secondary school students’ performance in Basic Science in Nigeria underscores the need for innovative instructional strategies. This study examined the impact of the Innovative Technology-Based Assessment Learning Package (ITBALP) on the achievement of JSS Two students in Ibadan, with gender as a moderating variable. Grounded in constructivist learning theory, the study used a quasi-experimental pretest-posttest control group design across six schools, with 150 students randomly selected. Schools were assigned to three groups: ITBALP with feedback and remediation (FR), ITBALP with feedback only, and a control group taught using conventional textbook methods. The Basic Science Achievement Test, validated with Cronbach’s alpha (r = 0.79), served as the instrument. Data were analyzed using ANCOVA at p = 0.05, and assumption checks confirmed robustness. Results showed a significant main effect of treatment on achievement (F(2,131) = 294.4, p < 0.05), with the FR group achieving the highest mean score (x̅ = 82.30), followed by the feedback group (x̅ = 63.04), and the control group (x̅ = 32.10). Gender had no significant main effect, and there was no treatment-by-gender interaction, indicating that ITBALP benefits both male and female students equitably. The findings underscore the value of integrating technology-enabled assessment with feedback and remediation to foster active, self-paced learning and improve science achievement. The study contributes to knowledge by operationalizing constructivist principles through digital assessment tools, offering evidence-based recommendations for teachers, curriculum planners, and policymakers to strengthen inclusive science education in Nigeria.