This dataset includes COVID-19 case and laboratory testing records compiled using the Final Line List and Laboratory Registration Format dated 25-11-2012 E.C. during the First Run. The data was collected by the Tigray Health Research Institute (THRI), based in Mekelle, Tigray, Ethiopia. Provided in Microsoft Excel (.xlsx) format, the dataset contains detailed patient information, including demographics, sample collection dates, test types, laboratory results, and clinical outcomes. The data collection follows standardized protocols to support accurate epidemiological surveillance and public health decision-making. Access to this dataset is governed by the Data Access and Sharing Procedure of the Research Data Management Committee (RDMC), ensuring responsible and ethical use.