Custom amplicon database for Shigella mPCR ARIBA screening

This dataset contains the FASTA sequences of PCR amplicons generated using the multiplex PCR primers described in Khokhar et al., "A low resource requirement molecular diagnostic and surveillance tool for Shigella in the era of vaccination" (submitted to Communications Medicine). The 15 amplicon sequences correspond to the Shigella serotyping targets (gtrI, gtrII, gtrX, oac-1b, oac-3a), species identification targets (ipaH, Shigella sonnei DNA methylase), antimicrobial resistance genes (blaCTX-M variants 1, 3, 14, 15, 27, 55; mph(A)), and the human RNaseP internal control. These sequences were used to construct a custom ARIBA database for in silico validation of mPCR amplicon specificity against whole genome sequencing data.