The dataset presented here was collected from biology teachers in Rwandan lower secondary schools who piloted a newly developed Virtual Science Laboratory (VSL) entitled Monohybridism with Complete Dominance Inheritance from April 23, 2024, to May 3, 2024. This VSL was developed by the Rwanda Quality Basic Education for Human Capital Development (RQBEHCD), a project financed by the World Bank Group through the Rwandan government.