Logo Lanfrica
  • Accueil
  • Atlas
  • Analyses
  • Documentation
  • Sign in

© 2026 Lanfrica. Tous droits réservés. Tous les droits d'auteur des ressources affichées sur le site Web Lanfrica appartiennent aux détenteurs de droits d'auteur d'origine, sauf indication contraire explicite.

Developing a flexible, high‐efficiency <i>Agrobacterium</i>‐mediated sorghum transformation system with broad application

Domaine:

agriculture

Type de record:

paper
Créateur:
PinAjiEmiJef
Éditeur:
WILEY
Hôte:
Summary Sorghum is the fifth most widely planted cereal crop in the world and is commonly cultivated in arid and semi‐arid regions such as Africa. Despite its importance as a food source, sorghum genetic improvement through transgenic approaches has been limited because of an inefficient transformation system. Here, we report a ternary vector (also known as cohabitating vector) system using a recently described pVIR accessory plasmid that facilitates efficient Agrobacterium ‐mediated transformation of sorghum. We report regeneration frequencies ranging from 6% to 29% in Tx430 using different selectable markers and single copy, backbone free ‘quality events’ ranging from 45% to 66% of the total events produced. Furthermore, we successfully applied this ternary system to develop transformation protocols for popular but recalcitrant African varieties including Macia , Malisor 84‐7 and Tegemeo . In addition, we report the use of this technology to develop the first stable CRISPR /Cas9‐mediated gene knockouts in Tx430.

Visit

doi.org

Licenses

http://creativecommons.org/licenses/by/4.0/

Similaires

Effect of type of mature embryo explants and acetosyringone on agrobacterium-mediated transformation of moroccan durum wheatGrowth Performance of Catfish (<i>Clarias gariepenus</i>) Cultured of High Density with Biofloc SystemDeveloping high-yielding <i>Striga</i> -resistant maize in sub-Saharan AfricaMulti‐Location Field Trials Revealed High‐Yielding and Broad‐Spectrum Resistance of Wheat ( <i>Triticum aestivum</i> L.) to Leaf Rust ( <i>Puccinia triticina</i> Eriks)Fermentability and nutritional characteristics of sorghum <i>Mahewu</i> supplemented with Bambara groundnutFlexible and cost-effective genomic surveillance of <i>P. falciparum</i> malaria with targeted nanopore sequencing

Effect of type of mature embryo explants and acetosyringone on agrobacterium-mediated transformation of moroccan durum wheat

Drought is one of the major constraints in durum wheat production in the Mediterranean Basin. In ord

Growth Performance of Catfish (<i>Clarias gariepenus</i>) Cultured of High Density with Biofloc System

Catfish is a species of freshwater fish that contains a source of protein and it has economic value

Developing high-yielding <i>Striga</i> -resistant maize in sub-Saharan Africa

Abstract Striga hermonthica is a majo

Multi‐Location Field Trials Revealed High‐Yielding and Broad‐Spectrum Resistance of Wheat ( <i>Triticum aestivum</i> L.) to Leaf Rust ( <i>Puccinia triticina</i> Eriks)

ABSTRACT Bread wheat plays a crucial role in global food an

Fermentability and nutritional characteristics of sorghum <i>Mahewu</i> supplemented with Bambara groundnut

Abstract Processing functionality in terms of fermentab

Flexible and cost-effective genomic surveillance of <i>P. falciparum</i> malaria with targeted nanopore sequencing

ABSTRACT Genomic surveillance of Plasmodium