Diagnostic performance of rKLO8 – and rK39 ELISA, rK39 strip test (ST) and DAT for visceral leishmaniasis in Sudan.

Abbreviations: TP, true positive; FN, false negative; TN, true negative; FP, false positive; PPV, positive predictive value; NPV, negative predictive value; CI, confidence interval; DAT, direct agglutination test; ITMA, Institute of Tropical Medicine Antwerp.

ELISA values were calculated combining results obtained at serum dilutions of 1∶800 and 1∶100. Detection of Leishmania in lymph node smears was used as reference. Specificity was calculated using 77 confirmed negative sera, including healthy controls, malaria -, TB-, and leukemia patients.