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Differential neurotoxicity of Southern African and Indian HIV-1 clade-C isolates.

Domaine:

healthcare
Créateur:
VasUjjEliVin
Hôte:avatar

A. gp120 of Indian HIV-1C isolates have minimal neurotoxicity. SH-SY5Y cells were incubated for 24 h with 50 µl of spent medium from PBMCs infected with representative isolates from (US HIV-1B, Southern African HIV-1C and Indian HIV-1C) with approximately similar viral loads. Supernatants were also immunodepleted of gp120 using anti-gp120 VRC03 (AIDS Repository) or Isotype Control IgG1 (BD) antibodies. WST-1 cell viability assay revealed US HIV-1B and Southern African HIV-1C supernatants caused significantly greater neuronal cell death compared to HIV-1C from India and a significant proportion of neuronal death is due to gp120. The absence of decrease in neurotoxicity upon neutralization of gp120 specifically in the Indian isolates was markedly different from the rest. Medium indicates untreated PBMC supernatants, while ‘Medium minus gp120’ refers to gp120 immunodepleted medium. An average of 2 Southern African isolates (medium) and of the 3 Indian isolates were compared. (*p<0.05; ***p<0.005).

Tags

Biological Sciences5yC 31S polymorphismgp 120gp 120 sequencesgp 120 ProteinTat dicysteine variantsneurotoxicityC 31 residueC 31S substitution+5

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CC BY 4.0

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