DNA probes for the identification of members of the Simulium damnosum complex (Diptera: Simuliidae)
Domaine:
healthcareCréateur:
R. P.
Éditeur:
Hôte:
Abstract
Genomic libraries in plasmid have been constructed from various sibling species of blackflies of the
Simulium damnosum
complex from West Africa. Three cloned repetitive sequences, which show variation in copy number between sibling species, have been isolated. These clones can be used as probes for the dot‐blot identification of larvae, pupae or adults into the three main West African subcomplexes, i.e.
damnosum, squamosum
and
sanctipauli
subcom‐plexes. The sequences also show some intraspecific variation in copy number.
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