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Dose-dependent RNAi-mediated geminivirus resistance in the tropical root crop cassava

Domaine:

agriculture

Type de record:

paper
Créateur:
Vanderschuren, HervéAlder, AdrianZhang, PengGruissem, Wilhelm
Éditeur:
ETHETH
Éditeur:
Springer
Hôte:avatar
Cassava mosaic disease is a major constraint for cassava production in Africa, resulting in significant economic losses. We have engineered transgenic cassava with resistance to African cassava mosaic virus (ACMV), by expressing ACMV AC1-homologous hairpin double-strand RNAs. Transgenic cassava lines with high levels of AC1-homologous small RNAs have ACMV immunity with increasing viral load and different inoculation methods. We report a correlation between the expression of the AC1-homologous small RNAs and the ACMV resistance of the transgenic cassava lines. Characterization of the small RNAs revealed that only some of the hairpin-derived small RNAs fall into currently known small interfering RNA classes in plants. The method is scalable to stacking by targeting multiple virus isolates with additional hairpins. © 2009 Springer Science+Business Media B.V.

Tags

Virus resistanceCassavaRNA interferenceHairpin RNA processing

Licenses

http://rightsstatements.org/page/InC-NC/1.0/In Copyright - Non-Commercial Use Permitted

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