This research examined the perceived effectiveness of a web-based application designed for nursing students to learn Ghanaian Sign Language. The study employed a descriptive survey design, utilising an online questionnaire deployed through the Kobo Toolbox application. The sample size for the study comprised of 133 student nurses selected through proportionate stratified sampling. The findings generally found the web-based application effective in learning Ghanaian Sign Language. They found the application’s navigation and interface user-friendly and visually appealing. The student nurses also asserted the Ghanaian Sign Language videos within the application were accurate and usable, enhancing the learning experience. However, students indicated challenges relating to the application loading times, data consumption, and the lack of an offline mode for the application. In light of these findings, it is recommended that the web-based application in Ghanaian Sign Language include technical optimisation, integrate an offline mode, and provide settings for data usage.