Gas Chromatography-Mass Spectrometry Analysis and Antidiabetic Effect of Fractionated Extract of Strophanthus hispidus

Domaine:

healthcareagriculture

Type de record:

paper

Créateur:

MuyTemAbiEst

Éditeur:

MJ&

Hôte:

Strophanthus hispidus DC (Apocynaceae) is a valued African plant that has been used as a remedy of various disease conditions. This study identified compounds present using GC-MS as well as the potential antidiabetic effects of the n-butanol, ethyl acetate, and aqueous fractions. Gas Chromatography Mass Spectrometry (GC-MS) was carried out using the Agilent 7890 A GC with a J&W HP-5MS column (30 m x 0.320 mm i.d. x 0.25 μm). The effects of the fractions (100 mg/kg each) on blood glucose levels, body weight, biochemical parameters, lipid profile, serum insulin, hepatic glycogen, and haemoglobin levels were evaluated in alloxan-induced hyperglycaemic rats. The GC-MS analysis revealed the presence of Decanoic acid methyl ester; methyl 15-acetoxy hexadecanoate; Hydrazine propyl; and Cyclobut-1-enyl methanol. A significant (p<0.05) reduction in blood glucose, creatinine, urea, bilirubin, liver enzymes, and glycosylated haemoglobin levels was observed with all fractions of S. hispidus administered compared to the untreated control. Also, an improvement in body weight, lipid profile, serum insulin, hepatic glycogen, and haemoglobin levels with S. hispidus fractions compared to the untreated control was observed. The study identifies the above-named compounds as potential antidiabetic agents in S. hispidus, with activity observed in both polar and non-polar media, warranting further investigation for compound isolation and characterization.

Simultaneous Determination of Selected Antidiabetic Pharmaceutical Drugs as Adulterants in Herbal Medicines Sold in Kenya Using Liquid Chromatography–Tandem Mass Spectrometry (LC–MS/MS)

A solid‐phase microextraction gas chromatography–mass spectrometry technique for urinary metabolomics of human samples infected with schistosomiasis—Case of the Okavango Delta, Botswana

Abstract We present a GC–MS metabolomics workflow for analyzing metabolites in urine samples infect

Gas Chromatography-Mass Spectrometry and Single Nucleotide Polymorphism-Genotype-By-Sequencing Analyses Reveal the Bean Chemical Profiles and Relatedness of Coffea canephora Genotypes in Nigeria

The flavor and health benefits of coffee (Coffea spp.) are derived from the metabolites that accumul

A validated liquid chromatography/tandem mass spectrometry method for the analysis of efavirenz in 0.2 mg hair samples from human immunodeficiency virus infected patients

Rationale Drug levels in hair provide a longer window of detection, compared t

Analysis of pesticide residues in potato varieties using modified quechers multi‐residue method combined with gas chromatography tandem mass spectrometric detection

Abstract Regular monitoring of pesticide residues in potato

Gas Chromatography Mass Spectroscopy (GC-MS) Characterization and Physicochemical Evaluation of DialiumGuineense (African Black Velvet Tamarind) Seed Oil

International audience Vegetable oils obtained from different oil plants are mainly u