Logo Lanfrica
  • Accueil
  • Atlas
  • Analyses
  • Documentation
  • Sign in

© 2026 Lanfrica. Tous droits réservés. Tous les droits d'auteur des ressources affichées sur le site Web Lanfrica appartiennent aux détenteurs de droits d'auteur d'origine, sauf indication contraire explicite.

Improving HIV-2 Detection by a Combination of Serological and Nucleic Acid Amplification Test Assays

Domaine:

healthcare

Type de record:

paper
Créateur:
AnnMicGiuRob
Éditeur:
Ame
Hôte:
ABSTRACT The ability to detect HIV-2 and to discriminate between HIV-1 and HIV-2 infections was evaluated in 46 serum samples from Guinea-Bissau (GB) and Guinea-Conakry (GC) using serological tests and commercial (HIV-1) and in-house (HIV-2) real-time PCR assays. Samples were first identified as HIV-2 positive by Genie I/II assay in GB and GC. HIV positivity was detected in 44 of 46 samples by all screening and confirmatory assays. A diagnostic strategy based on Inno-LIA and HIV-1/2 RNA detection assays allowed accurate discrimination between HIV-1 and HIV-2 in 84% of single infections and confirmed 32% of double infections. In samples with double reactivity in the Inno-LIA test and no detection of both genomes, cross-reactivity likely hampered the identification of true double infections. In conclusion, the implementation of a diagnostic strategy, based on multiple specific serological tests and highly sensitive quantitative PCR assays, is recommended to ensure accurate HIV-2 diagnosis and appropriate therapy for individuals from areas in which the virus is endemic.

Visit

doi.org

Licenses

https://journals.asm.org/non-commercial-tdm-license

Similaires

Silaffin Tagged Polymerase for Nucleic Acid AmplificationNucleic acid detection and quantification in the developing worldNucleic acid sequences.Reduction of the risk of transfusion‐transmitted viral infection by nucleic acid amplification testing in the Western Cape of South Africa: a 5‐year reviewDetection of strains of African cassava mosaic virus by nucleic acid hybridisation and some effects of temperature on their multiplicationAssessment of HIV transfusion transmission risk in South Africa: a 10‐year analysis following implementation of individual donation nucleic acid amplification technology testing and donor demographics eligibility changes

Silaffin Tagged Polymerase for Nucleic Acid Amplification

There is an ever-growing demand for diagnostic relevant biological reagents. However, the high cost

Nucleic acid detection and quantification in the developing world

Techniques using nucleic acid amplification have not had the same amount of impact on research and c

Nucleic acid sequences.

Animal Trypanosomosis (AT) is a significant disease affecting cattle across sub-Saharan Afri

Reduction of the risk of transfusion‐transmitted viral infection by nucleic acid amplification testing in the Western Cape of South Africa: a 5‐year review

Background and Objectives  In October 2005, individual donation nucleic acid amplification testing

Detection of strains of African cassava mosaic virus by nucleic acid hybridisation and some effects of temperature on their multiplication

SUMMARY DNA probes, made by cloning double‐stranded forms of each of the genome parts (DNA‐1 and DN

Assessment of HIV transfusion transmission risk in South Africa: a 10‐year analysis following implementation of individual donation nucleic acid amplification technology testing and donor demographics eligibility changes

BACKGROUND In 1998 we estimated that 34/million infectious window period donations were entering th