This release provides the set of scripts used for RNA-seq data processing and downstream analysis described in the manuscript "Conserved neutrophil degranulation transcripts in HIV–TB coinfected children across East and Southern Africa."
The repository includes scripts for:
FASTQ quality control of raw RNA-seq data
Transcript quantification using Salmon
Differential expression analysis using DESeq2
Construction of protein–protein interaction (PPI) networks
These analyses were performed on bulk RNA-seq data from children living with HIV in Uganda, Botswana, and Eswatini as part of the CAfGEN consortium study.