The aim of our study is to check the performance of the new 100% Moroccan SARS-CoV-2 detection kit: MAScIR SARS-CoV-2 M 2.0. The following parameters were studied: repeatability, intermediate precision, specificity, sensitivity, contamination test, and comparison with the GeneFinderTM COVID-19 Plus RealAmp kit. In addition, an external quality evaluation comprising five samples was carried out. During our study, we also performed sequencing of some randomly selected positive samples, among which we detected carriers of the Alpha variant, the Delta variant, and the Omicron variant.