The phylogenetic tree illustrates the evolutionary relationships among various MPXV isolates collected between 2022 and 2024. The tree including 118 sequences from different geographical locations, primarily Nigeria, South Africa, Cameroon, Egypt, Sudan, and the Democratic Republic of the Congo downloaded and analysis by National Center for Biotechnology Information (NCBI). The isolates are grouped into two main clades: Clade II (Black), corresponding to the period from May 2022 to May 2023, and Clade Ib (Red), representing the interval from October 2023 to January 2024. This classification reflects distinct evolutionary lineages.
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