PHYTOCHEMICAL, TOXICOLOGICAL, AND PHARMACOLOGICAL INVESTIGATION OF INDIGENOUS MEDICINAL PLANTS FROM ONDO/OGUN STATES FOR ANTI‑MALARIA DRUG DISCOVERY

Malaria remains a major public health challenge in sub-Saharan Africa, compounded by increasing resistance of Plasmodium falciparumto existing anti-malarial therapies. This study investigated the phytochemical composition, toxicological safety, and pharmacological efficacy of selected indigenous medicinal plants from Ondo and Ogun States, Southwest Nigeria, traditionally used for malaria treatment. An ethnobotanical survey guided plant selection, followed by solvent extraction, phytochemical screening, chromatographic isolation, and spectroscopic characterisation of bioactive compounds. In-vitro anti-plasmodial activity was evaluated against chloroquine-resistant P. falciparumstrains, while in-vivo efficacy was assessed using Plasmodium berghei-infected albino mice. Acute toxicity studies were conducted in accordance with OECD guidelines. Results revealed that several plant extracts, particularly Morinda lucida, Enantia chlorantha, and Vernonia amygdalina, exhibited strong anti-plasmodial activity with IC₅₀ values below 12 μg/mL and significant parasite suppression in vivo. Toxicological findings indicated wide safety margins with no severe adverse effects at therapeutic doses. The study validates indigenous anti-malarial knowledge and identifies promising plant-derived candidates for further drug development. These findings highlight the potential of locallysourced medicinal plants as affordable and culturally acceptable leads in the search for new anti-malarial drugs.