Strategy employed for PCR-based karyotyping of the 2Rj inversion

Copyright information:

Taken from "PCR-based karyotyping of inversion 2identifies the BAMAKO chromosomal form"

malariajournal.com

Malaria Journal 2007;6():133-133.

Published online 1 Oct 2007

PMCID:PMC2134931.

At top, schematic representation of 2R (black line), indicating approximate position of inversions j, b, c and u; asterisk indicates centromere. Below, schematic representation of primer binding sites on the standard (+) and inverted (j) chromosomes (hatched and shaded rectangles). The relevant portion of the cytogenetic map is presented below the standard chromosome. Numbers above each chromosome refer to different breakpoint junctions. Double-headed arrow between breakpoint junctions of the inverted chromosome represents an identical 14.6 kb insertion containing 5.3 kb inverted repeats separated by a 4 kb spacer. Labeled arrows above and below chromosomes indicate primer pairs targeting the standard and inverted arrangement of 2Rj, respectively; expected length of the PCR product in bp given between the arrows. Chromosome segments and primers are not to-scale.