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Use of monoclonal antibody to potato leafroll virus for detecting groundnut rosette assistor virus by ELISA*

Domaine:

agriculture

Type de record:

paper
Créateur:
R. A. THE
Éditeur:
WILEY
Hôte:
SUMMARY Three of 10 monoclonal antibodies (MAbs) produced to potato leafroll luteovirus (PLRV) were found to react in triple antibody sandwich ELISA (TAS‐ELISA) with groundnut rosette assistor luteovirus (GRAV), though none reacted with four other luteoviruses (barley yellow dwarf, bean leaf roll, beet western yellows or carrot red leaf)‐ The most effective PLRV MAb, SCR 6, was used in TAS‐ELISA to detect isolates of GRAV from groundnut plants with chlorotic, green and mosaic forms of rosette from Nigeria and Malawi. The test also detected GRAV in extracts of single Aphis craccivora.

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