Additional file 1 of Development of a new barcode-based, multiplex-PCR, next-generation-sequencing assay and data processing and analytical pipeline for multiplicity of infection detection of Plasmodium falciparum

Additional file 1: Figure S1. RF classification using laboratory strains. Figure shows proportion of each unique amplicon relative to all amplicons from the targeted region on a plate from all laboratory strain samples based on RF classifier (when random selection determined the training set). X axis is the log10 count of SNP-specific reads in a Miseq run and y axis is log10 count of unique amplicon-specific reads in that individual run. Red points are amplicons classified as negative (False) for exclusion while green points are amplicons classified as positive (True) for further SNP frequency analysis.