Additional file 5 of Development of a new barcode-based, multiplex-PCR, next-generation-sequencing assay and data processing and analytical pipeline for multiplicity of infection detection of Plasmodium falciparum

Additional file 5: Figure S5. StrainRecon reconstruction on laboratory-mixed samples given advance knowledge of n = 3 strains. Ground truth of barcodes (first row in each figure), the MAP estimate of the reconstruction matrix M and mixture vector v (second row), and the mean (third row) and standard deviation (fourth row) of the posterior density of candidate (M, v) solutions. Each block contains one row for each strain, ordered by decreasing frequency from top, with a SNP cell color ranging from purple (fraction of 0) to bright yellow (fraction of 1). The figure showcases the algorithm outputs with n = 3 strains on a variety of samples and mixtures, including cases of accurate and unique strain reconstruction (such as B33_PA_DBS).